Hplc Analysis And Quantification Of Prepared Samples Training

Learning Outcomes

• Apply HPLC principles and instrumentation to prepare and analyze samples according to standard operating procedures.
• Demonstrate correct calibration and operation of HPLC systems, including pump, injector, column, and detector components.
• Analyze chromatographic data to identify and quantify target analytes using appropriate software and calculations.
• Evaluate the quality of HPLC results by performing system suitability tests and troubleshooting common issues.
• Implement safety and quality assurance protocols in the HPLC laboratory to ensure reliable and reproducible outcomes.
• Document analytical results and maintain records in compliance with laboratory standards and regulatory requirements.

Target Audience

This course is intended for laboratory technicians, analysts, and quality control personnel who are involved in HPLC analysis and require formal recognition of their competencies. It is also suitable for individuals seeking to upskill in chromatographic techniques within pharmaceutical, chemical, food, or environmental testing laboratories.

Prerequisites

None — open enrollment.

Course Outline

Practicals

Overview

Hands-on practical sessions are essential for learners to gain proficiency in HPLC operation, sample preparation, method development, and data analysis. These sessions allow learners to apply theoretical knowledge in a controlled laboratory environment, ensuring they can independently perform HPLC analysis and quantification.

Practical Activities

• HPLC Instrument Familiarization and Operation — Learners will identify HPLC modules, perform start-up and shutdown, set up a simple isocratic method, and inject standards. (8h)
• Sample Preparation and Calibration — Learners will prepare samples using filtration and dilution, create calibration standards, and construct calibration curves. (8h)
• Method Development and Optimization — Learners will develop a simple reversed-phase method, optimize mobile phase composition and flow rate, and perform system suitability tests. (8h)
• Full Analysis Workflow and Assessment — Learners will complete a full analysis from sample preparation to final report, including troubleshooting and data interpretation. (8h)

Summatives

Each delegate is assessed continuously throughout the course via daily exercises, scored practical assignments, and a final summative test at the end.

Practical Assignments — 30%

Practical assignments are observed and scored against a rubric during the practical sessions. Each delegate's practical mark is averaged into a single 100% score and contributes 30% to the final total.

Daily Exercises — 20%

Every training day ends with a multiple-choice exercise scored out of 100%. The scores from each daily exercise are averaged across the duration of the course to produce a Daily Average mark, which contributes 20% to the final total.

Final Test — 50%

On the last day a final summative test is written. It is a multiple-choice paper with multiple-answer questions: each question may have more than one correct option, and a single wrong selection on a question marks the entire question wrong — no partial credit. The final test is scored out of 100% and contributes 50% to the overall mark.

Final Total

Component	Out of	Weight
Practical Assignments (rubric-scored)	100%	30%
Daily Average (multiple choice)	100%	20%
Final Test (multi-answer multiple choice)	100%	50%
Final Total	—	100%

All marks are recorded on the AATICD LMS and visible to each learner under their account.

Certificate

How it works

• Certificates are auto-generated on the AATICD LMS as soon as the marks pass the 50% threshold.
• Each certificate is a branded PDF with the delegate's name, the course title, the unit standard ID, NQF level, credits, and the date of issue.
• You can download or print your certificate from your LMS dashboard at any time after issue — there's no reissue fee and no expiry date.
• If you scored under 50% you can sit the final test again at the next scheduled session at no extra cost.

Where to find it

Sign in to the LMS, open your dashboard, and your certificates appear under My Certificates. Each entry has a View / Download button and a print option.

Training Discounts

Group discounts apply automatically — the more delegates you enrol, the greater the saving. Discounts are calculated at 3% per 5 delegates, scaling up to 40% off for 100+ delegates.

Delegates	Discount
5	3% off
10	6% off
15	9% off
20	12% off
25	15% off
30	18% off
50	30% off
75	35% off
100	40% off

3% discount per 5 delegates, up to 40% off for 100+ delegates. Contact us for a custom group quote.