Health Sciences and Social Services Curative Health

Blood Product Fractionation for Transfusion Training

SAQA US 257199 | NQF 3 | Credits 12 | Duration 9 Days
From $1,318 per delegate

Description

This course equips learners with the knowledge and skills required to fractionate blood products for transfusion, ensuring safe and effective processing of whole blood into components such as red cells, platelets, plasma, and cryoprecipitate. It covers the principles of blood component preparation, quality control, and storage to meet South African transfusion standards.

Learning Outcomes

  • Apply standard operating procedures for the separation of whole blood into components using centrifugation and other fractionation techniques.
  • Demonstrate the correct labeling, storage, and transportation of blood products to maintain integrity and traceability.
  • Evaluate quality control parameters for each blood component to ensure compliance with regulatory requirements.
  • Implement safety protocols to minimise risks of contamination, haemolysis, and adverse transfusion reactions.
  • Analyse the indications and contraindications for the use of different blood components in clinical practice.
  • Document fractionation processes accurately in accordance with laboratory information systems and quality management standards.

Target Audience

This course is designed for medical laboratory technologists, blood bank technicians, and healthcare professionals involved in blood transfusion services who require competence in blood product fractionation.

Prerequisites

None — open enrollment.

Course Outline

Day 1: Introduction to Blood Products and Transfusion Safety

Objectives:
• Understand the composition and types of blood products used in transfusion.
• Explain the risks and safety measures in blood transfusion.
• Describe the regulatory framework governing blood product fractionation.
• Identify key stakeholders and their roles in the transfusion chain.

Topics:
• Overview of whole blood and its components (RBCs, platelets, plasma, cryoprecipitate)
• Indications for transfusion of different blood products
• Transfusion-transmissible infections and donor screening
• Regulatory bodies: SAQA, SANBS, HPCSA, and relevant legislation
• Quality management systems in blood product processing

Day 2: Principles of Plasma Fractionation

Objectives:
• Describe the plasma fractionation process and its history.
• Differentiate between various fractionation methods.
• Explain the role of anticoagulants and preservatives.
• Identify the main plasma-derived products (albumin, immunoglobulins, clotting factors).

Topics:
• Historical development of plasma fractionation (Cohn process)
• Cold ethanol fractionation method
• Chromatographic techniques (ion exchange, affinity, size exclusion)
• Viral inactivation and removal methods (solvent/detergent, nanofiltration, pasteurization)
• Product portfolio: albumin, IVIG, Factor VIII, Factor IX, fibrinogen

Day 3: Red Blood Cell and Platelet Processing

Objectives:
• Explain the steps in red blood cell concentrate preparation.
• Describe platelet concentrate production methods (PRP, buffy coat, apheresis).
• Understand storage conditions and shelf life for RBCs and platelets.
• Discuss quality control parameters for cellular components.

Topics:
• Whole blood collection and component separation (centrifugation)
• Additive solutions for RBC preservation (SAG-M, AS-1)
• Platelet production: random donor vs. apheresis
• Platelet storage: temperature, agitation, and bacterial screening
• Quality testing: hematocrit, platelet count, sterility, hemolysis

Day 4: Cryoprecipitate and Plasma Derivatives

Objectives:
• Describe the preparation of cryoprecipitate and its clinical use.
• Explain the production of fresh frozen plasma (FFP) and pathogen-reduced plasma.
• Understand the role of cryoprecipitate in fibrinogen replacement.
• Differentiate between single-donor and pooled plasma products.

Topics:
• Cryoprecipitate production: thawing, centrifugation, and freezing
• Indications for cryoprecipitate: hemophilia A, von Willebrand disease, hypofibrinogenemia
• FFP and solvent/detergent-treated plasma (Octaplas)
• Pooling and fractionation of plasma for industrial production
• Lyophilization and storage of plasma derivatives

Day 5: Pathogen Reduction and Inactivation Technologies

Objectives:
• Explain the need for pathogen reduction in blood products.
• Describe common pathogen reduction technologies for platelets and plasma.
• Understand the impact of pathogen reduction on product quality.
• Discuss regulatory requirements for pathogen-reduced products.

Topics:
• Overview of pathogen reduction systems: INTERCEPT (psoralen + UVA), Mirasol (riboflavin + UV), THERAFLEX (methylene blue)
• Mechanism of action: nucleic acid crosslinking
• Effect on platelet function and plasma proteins
• Pathogen reduction for RBCs: S-303 and amustaline
• Validation and quality control for pathogen reduction

Day 6: Fractionation of Granulocytes and Special Products

Objectives:
• Describe granulocyte collection and processing for transfusion.
• Explain the production of convalescent plasma and hyperimmune globulins.
• Understand the fractionation of cord blood stem cells.
• Discuss emerging blood products (e.g., whole blood for trauma).

Topics:
• Granulocyte apheresis and donor stimulation with G-CSF
• Convalescent plasma: collection, pathogen reduction, and indications
• Hyperimmune globulins: hepatitis B, rabies, tetanus
• Cord blood processing and cryopreservation
• Low-titer group O whole blood for military and trauma settings

Day 7: Quality Control and Assurance in Fractionation

Objectives:
• Outline the quality control tests for each blood product.
• Explain the role of Good Manufacturing Practice (GMP) in fractionation.
• Understand the process validation and batch release.
• Discuss adverse event reporting and traceability.

Topics:
• QC testing: sterility, potency, purity, and stability
• GMP requirements: clean rooms, environmental monitoring, personnel hygiene
• Process validation: viral validation studies, hold time studies
• Batch release procedures and lot number traceability
• Haemovigilance and error management

Day 8: Storage, Distribution, and Logistics of Blood Products

Objectives:
• Explain the cold chain requirements for different blood products.
• Describe inventory management and blood ordering systems.
• Understand the logistics of transporting blood products.
• Discuss wastage reduction strategies.

Topics:
• Temperature monitoring: refrigerators, freezers, transport containers
• Inventory management: FIFO, cross-matching, reservation
• Blood delivery: transport validation, temperature excursion management
• Wastage: causes, prevention, and reporting
• Disaster preparedness and emergency blood supply

Day 9: Clinical Application and Integration

Objectives:
• Apply knowledge of blood products to clinical scenarios.
• Interpret blood product labels and expiry dates.
• Discuss appropriate selection of products for specific patients.
• Understand the role of fractionation in rare blood types and special needs.

Topics:
• Case studies: massive transfusion protocol, neonatal transfusion, immune deficiency
• Product selection: matching patient needs to available components
• Special considerations: irradiated, washed, CMV-negative products
• Emerging trends: artificial blood substitutes, recombinant factors
• Final assessment and course wrap-up

Practicals

27 hours of practicals To be conducted online or on-campus or in-house
Overview

Hands-on practicals are essential for learners to apply fractionation techniques in a simulated laboratory environment. These sessions cover component separation, pathogen reduction procedures, and quality control testing to reinforce theoretical knowledge and ensure competency in safe blood product preparation.

Practical Activities
  • Whole Blood Component Separation — Learners perform centrifugation and separation of whole blood into packed red cells, buffy coat, and plasma using standard blood bank equipment. (6h)
  • Plasma Fractionation by Cold Ethanol Method — Learners simulate the Cohn fractionation process using a scaled-down method to isolate albumin and immunoglobulin fractions from plasma. (6h)
  • Pathogen Reduction of Platelets — Learners apply a pathogen reduction system (e.g., INTERCEPT) to platelet concentrates, following manufacturer instructions for illumination and storage. (6h)
  • Quality Control Testing of Blood Products — Learners conduct routine QC tests on prepared blood components, including hematocrit, platelet count, and sterility testing. (6h)
  • Simulated Batch Release and Traceability Exercise — Learners simulate the batch release process by reviewing QC data, verifying labels, and completing release documentation for a batch of blood products. (3h)

Summatives

Each delegate is assessed continuously throughout the course via daily exercises, scored practical assignments, and a final summative test at the end.

Practical Assignments — 30%

Practical assignments are observed and scored against a rubric during the practical sessions. Each delegate's practical mark is averaged into a single 100% score and contributes 30% to the final total.

Daily Exercises — 20%

Every training day ends with a multiple-choice exercise scored out of 100%. The scores from each daily exercise are averaged across the duration of the course to produce a Daily Average mark, which contributes 20% to the final total.

Final Test — 50%

On the last day a final summative test is written. It is a multiple-choice paper with multiple-answer questions: each question may have more than one correct option, and a single wrong selection on a question marks the entire question wrong — no partial credit. The final test is scored out of 100% and contributes 50% to the overall mark.

Final Total
Component Out of Weight
Practical Assignments (rubric-scored) 100% 30%
Daily Average (multiple choice) 100% 20%
Final Test (multi-answer multiple choice) 100% 50%
Final Total 100%

All marks are recorded on the AATICD LMS and visible to each learner under their account.

Certificate

Certificate of Completion

Awarded to delegates who achieve an overall mark of 50% or higher on the Final Total (Practicals 30% + Daily Average 20% + Final Test 50%).

How it works
  • Certificates are auto-generated on the AATICD LMS as soon as the marks pass the 50% threshold.
  • Each certificate is a branded PDF with the delegate's name, the course title, the unit standard ID, NQF level, credits, and the date of issue.
  • You can download or print your certificate from your LMS dashboard at any time after issue — there's no reissue fee and no expiry date.
  • If you scored under 50% you can sit the final test again at the next scheduled session at no extra cost.
Where to find it

Sign in to the LMS, open your dashboard, and your certificates appear under My Certificates. Each entry has a View / Download button and a print option.

Training Discounts

Group discounts apply automatically — the more delegates you enrol, the greater the saving. Discounts are calculated at 3% per 5 delegates, scaling up to 40% off for 100+ delegates.

Delegates Discount
5 3% off
10 6% off
15 9% off
20 12% off
25 15% off
30 18% off
50 30% off
75 35% off
100 40% off

3% discount per 5 delegates, up to 40% off for 100+ delegates. Contact us for a custom group quote.

Upcoming Training Sessions
Online training — attend live sessions from anywhere via our virtual classroom.
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Training Discounts
Delegates Discount
5 3% off
10 6% off
15 9% off
20 12% off
25 15% off
30 18% off
50 30% off
75 35% off
100 40% off

3% off per 5 delegates, up to 40% for 100+

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